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KMID : 0380620070390010077
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 1 p.77 ~ p.82
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Nitrite Scavenging Ability and SOD-Like Activity of a Sterol Glucoside from Chrysanthemum coronarium L. var. spatiosum
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Cho Min-Jung
Park Mi-Jung
Lee Heum-Sook
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Abstract
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From the total methanolic extract of Chrysanthemum coronarium L. var. spatiosum (Compositae), nitrite scavenging ability and superoxide dismutase (SOD)-like activity were analyzed as antioxidative characteristics. After successive partitioning with chloroform, n-butanol, and water, the chloroform fraction showed the most significant nitrite scavenging ability with an value of 39 ppm compared with the values of vitamin C and chlorogenic acid, 15 ppm and 36 ppm, respectively. The active fraction was subjected to silica gel and Sephadex LH-20 column chromatography, and the compound was isolated and identified as using and spectral data. The glucoside was further hydrolyzed and confirmed as a glycosylated . The compound and its aglycone, , showed different nitrite scavenging and SOD-like activity. The value of nitrite scavenging ability of the compound was 335 ppm at pH 1.5, while that of its aglycone was 41 ppm. As for the SOD-like activity, the values of the sterol and the glucoside were 1,291 ppm and >2,000 ppm, respectively, compared with those of vitamin C and chlorogenic acid, 38 ppm and 449 ppm, respectively.
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KEYWORD
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Chrysanthemum coronarium L. var. spatiosum, Compositae, β-sitosterol-Ο-β-D-glucoside, nitrite scavenging ability, SOD-Like activity
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